FIG. 4.

Analysis of the conserved promoter-like sequence between YMTV23.5L and YMTV24L. (a) Alignment of a conserved sequence found between orthologs of YMTV23.5L and YMTV24L. The sequence within the red boxes labeled “A” and “B” represents the 9-bp repeat. The numbers above the grey box indicate the nucleotide positions. (b) Schematic of the orientation of the promoterless GFP with respect to the orientation of the cloned myxoma virus conserved sequence. R-GFP contains the conserved sequence from bases 2 to 41. L-GFP contains the reverse complement of the conserved sequence. The red boxes show the location of the two repeats in panel a. (c) Cells were either mock infected or infected with myxoma virus and subsequently transfected with either a promoterless GFP, R-GFP, or L-GFP construct. Forty-eight hours postinfection, the cells were visualized using a fluorescence microscope.