Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
(A) An image of primer extension on primers 1 and 4 at increasing concentrations of 0.5, 1, 2, 3, 4, 5, 6, 8, 10, 15 and 20 µM. The quantum yields of fluorescence emission of Cy5-labeled template is 3.3 times that of Cy5-ddGTP in aqueous solution. (B) A plot of relative fluorescence intensity of the Cy5-ddGTP-labeled primers versus concentration. Each data point is the average of the integral of five spots. Filled squares, primer 1; filled diamonds, primer 4.
