Plastid vectors to compare
translation efficiency from neo mRNAs with the E.coli DB and plastid DS regions. (A)
DNA sequence of Prrn promoters with T7g10 leader derivatives. The
Prrn promoter region is underlined; the transcription initiation
site is marked by a horizontal arrow; the translation initiation
codon (ATG) is in bold; the SD sequence is underlined with a wavy
line. The T7g10 DB (Ec) sequence is shown on top;
the sequence with the plastid DS region (pt) is duplicated in the
middle; the sequence with the NheI site is at the
bottom. Plastid and T7g10 sequences are in capital letters; nucleotides
added or modified during construction are in lowercase; gaps are
marked with dashes. (B) The plastid targeting region
of the transformation vectors. The spectinomycin resistance (aadA)
and neo genes and plastid genes rrn16, trnV and rps12/7 (46)
are shown. The positions of the neo promoter (Prrn)
and 3′-UTR (TrbcL) are also marked.
The wavy line represents 1.0 kb neo mRNA. Abbreviations
for restriction sites: E, EcoRI; S, SacI;
N, NheI; X, XbaI; H, HindIII;
B, BglII. Restriction sites removed during plasmid
construction are in parentheses. (C) Listing
of plasmids and the schematic map of their promoter and N-terminal coding
regions. For an explanation see (B).