Table 1. Results of kinetic measurements and analysis.

		32 base hairpin capture probe			16 base hairpin capture probe
Temperature	[Target]a	k × [T0]b	Ratec	R2	k × [T0]b	Ratec	R2
25°C	0.001	1.1 ± 0.2	0.23 ± 0.02	0.9250	0.53 ± 0.08	0.11 ± 0.02	0.9175
	0.004	4.9 ± 1.0	1.1 ± 0.3	0.8882	2.2 ± 0.1	0.44 ± 0.07	0.8874
	0.016	19 ± 3	4.2 ± 1.2	0.9441	8.6 ± 1.3	1.7 ± 0.2	0.9414
	0.064	64 ± 9	14 ± 3	0.9633	53 ± 6	11 ± 1	0.9850
45°C	0.001	4.2 ± 1.0	0.90 ± 0.09	0.9820	1.7 ± 0.2	0.33 ± 0.05	0.8547
	0.004	19 ± 4	4.0 ± 0.5	0.9796	6.1 ± 1.0	1.2 ± 0.2	0.8367
	0.016	70 ± 3	15 ± 1	0.9970	28 ± 4	5.6 0.5	0.9097
	0.064	270 ± 10	58 ± 3	0.9986	82 ± 8	17 ± 2	0.9854
		32 base linear capture probe			16 base linear capture probe
25°C	0.001	ND	ND	ND	0.15 ± 0.03	0.048 ± 0.009	0.8764
	0.004	1.8 ± 0.5	0.58 ± 0.03	0.8983	0.62 ± 0.08	0.20 ± 0.02	0.8983
	0.016	9.0 ± 3.0	2.8 ± 0.4	0.8158	2.6 ± 0.4	0.82 ± 0.08	0.9665
	0.064	23 ± 2	7.3 ± 0.8	0.9826	8.8 ± 0.9	2.8 ± 0.3	0.9690
45°C	0.001	1.0 ± 0.2	0.31 ± 0.05	0.9935	0.84 ± 0.2	0.27 0.05	0.7405
	0.004	4.9 ± 1.1	1.5 ± 0.8	0.9743	2.1 ± 0.2	0.69 ± 0.1	0.8491
	0.016	18 ± 2	5.6 ± 2.9	0.9828	8.6 ± 0.9	2.8 ± 0.4	0.8856
	0.064	64 ± 11	20 ± 3	0.9854	26 ± 3	8.4 ± 0.8	0.9615

^a10^–8 M strands.

^b10^–9/s (k and [T₀] were determined from fits to equation 6).

^cEvaluated from k × [T₀] × [P₀], 10^–12 M/s. See text for details.

ND, not determined.