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. 2001 Feb 15;29(4):904–913. doi: 10.1093/nar/29.4.904

Figure 2.

Figure 2

Purification of recombinant ORF56. Protein from various steps of the purification protocol was analyzed on a 16% SDS–Tris–Tricine polyacrylamide gel (12) stained with Coomassie Blue. Lane 1, whole cell extract of an uninduced control culture; lane 2, whole cell extract of an induced E.coli (pET28-orf56) culture; lane 3, crude extract (centrifuged whole cell extract) of an induced culture; lane 4, heat step supernatant; lanes 5–7, pooled peak fractions of purified ORF56; lanes M1 and M2, molecular mass markers.