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Chemical crosslinking of ORF56 with DMS. ORF56 was incubated with and without crosslinker and analyzed on a denaturing peptide gel. Lane 1, no DMS; lane 2, 10 mM DMS added. The crosslinking reaction was repeated in the presence of binding site DNA (wt): lane 3, 10 mM DMS; lane 4, no DMS.
