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Thermal denaturation of wt+CGC DNA in the absence and presence of ORF56. The temperature profiles of 1 µM wt+CGC DNA (open circles) and of 1 µM wt+CGC DNA and 4 µM ORF56 (closed circles) are shown. Every second data point is presented. The melting temperature Tm was obtained by fitting according to equation 5 (see Materials and Methods). Tm was 71 and 86°C, respectively.
