Table 1. Synthetic DNA substrates used to evaluate the DNA-binding activity of ORF56.

Alignment of the synthetic DNA substrates used in this study. wt is derived from the longer inverted repeat within the promoter region of gene orf56 (Fig. 5). wt+CGC has three additional bases at both termini in order to increase the melting temperature of the corresponding duplex DNA. rep is the half-site sequence of the wt DNA. The synthetic substrate inv is derived from the shorter inverted repeat within the promoter region. ct is a control DNA used in the study. The arrows indicate repeat structures. Only one strand is shown. Double-stranded DNAs were used in all experiments.