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(A) Sequence and cloverleaf structure of E.coli tRNACys, where the boxed U73 is the major determinant for aminoacylation with cysteine. (B) Sequence and secondary structure of the synthetic E.coli microhelixCys, where U73 is boxed. (C) Sequence and secondary structure of the synthetic M.pneumoniae microhelixCys, where U73 is boxed. (D) Sequence and cloverleaf structure of B.subtilis tRNACys. The arrow indicates separation of the 5′- and 3′-fragments for reconstitution.
