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Imino spectrum at 15°C of the M.pneumoniae microhelix tRNACys. The assignments are indicated above their corresponding resonance position. The imino proton resonances for U8 and G11 confirm that a UUCG tetraloop is formed. The presence of an imino peak for G1 is exceptional at this high a temperature and supports the structural model wherein stacking of A76 onto G1 serves to protect the G1 imino proton from exchange.
