Figure 2.

Bifunctionality of R.BseMII. Lane 1, λ DNA; lane 2, λ DNA incubated with 5 U of R.BseMII in a reaction mixture containing 10 mM Mg²⁺ and 10 µM AdoMet; lane 3, λ DNA incubated with 5 U of R.BseMII in reaction mixture without Mg²⁺ and AdoMet and, following addition of Mg²⁺ and AdoMet, cleaved with R.BseMII; lane 4, λ DNA incubated with 5 U of R.BseMII in a reaction mixture with 10 µM AdoMet without Mg²⁺ and, following addition of Mg²⁺, cleaved with R.BseMII; lane 5, λ DNA incubated with 5 U of R.BseMII in the reaction mixture with 10 µM AdoMet without Mg²⁺ and, following addition of Mg²⁺ and 1 µg λ DNA, cleaved with R.BseMII.