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Assay of the cofactors that are required in R.BseMII cleavage reaction. Lane 1, λ DNA; lane 2, λ DNA digested with 5 U of R.BseMII in the presence of 1 mM ATP, 20 µM AdoMet and 10 mM Mg2+; lane 3, same components as in lane 2 without ATP; lane 4, same components as in lane 3 without AdoMet; lane 5, same components as in lane 4 without Mg2+.
