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TNFα protein production is differentially regulated in peritoneal macrophages (activated with IFNγ and/or LPS) isolated from both B10.A and NZW mice. ELISA analysis was performed with supernatants obtained from peritoneal macrophages stimulated with LPS and/or IFNγ for 16 h. Supernatants obtained from untreated cells were used as a control. The concentrations of the stimuli were as follows: LPS, 1 µg/ml; IFNγ, 100 U/ml.
