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. 2001 Feb 15;29(4):863–871. doi: 10.1093/nar/29.4.863

Figure 4.

Figure 4

Figure 4

Binding of macrophage proteins to the TNFα ARE is hindered by a GAU trinucleotide insertion. RNA-binding assays were performed with 100 000 c.p.m. of labeled TNF-10 and TNF-10m (containing GAU insertion) cRNA probes, alone (lanes 1 and 3) or with 10 µg of TCE obtained from macrophage cells (lanes 2 and 4) in the absence of RNase T1. The RNA–protein complexes were then UV-crosslinked for 8 min and the resulting complexes were analyzed by non-denaturing gel (A) or by SDS–PAGE gels (B). A, B and C denote the positions of the main RNA–protein complexes. F denotes free probe and free nucleotides.