Skip to main content
. 2001 Feb 15;29(4):863–871. doi: 10.1093/nar/29.4.863

Figure 5.

Figure 5

Figure 5

Binding of macrophage proteins to the TNFα ARE is hindered by a CAU trinucleotide insertion. cRNA labeled TNF-10 and TNF-10m2 (CAU insertion) probes (100 000 c.p.m.) were incubated alone (lanes 1 and 3) or with 10 µg of TCE prepared from untreated murine macrophages (lanes 2 and 4). The resulting RNA–protein complexes were treated with RNase T1, UV-crosslinked and analyzed on non-denaturing polyacrylamide gel (A) as well as on SDS–PAGE gels (B). A, B and C denote the positions of the main RNA–protein complexes. F denotes free probe, free nucleotides and remnants of undigested RNA.