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. 2001 Feb 15;29(4):863–871. doi: 10.1093/nar/29.4.863

Figure 6.

Figure 6

Figure 6

Actinomycin D induces the formation of complex B and C with TNFα mRNA. Probe TNF-10 was incubated alone (lane 1) or with protein extracts prepared from B10R cells untreated (lanes 2, 4, 6 and 8) or treated with 5 µg/ml of Actinomycin D for 1 h (lanes 3, 5, 7 and 9). Lanes 2 and 3, total cellular extract; lanes 4 and 5, nuclear wash fraction; lanes 6 and 7, nucleoplasm; lanes 8 and 9, total cytoplasmic extract. The resulting RNA–protein complexes were analyzed either on the non-denaturing gel (A) or UV-crosslinked and analyzed on denaturing 8% polyacrylamide–SDS gel (B).