Figure 1.
The T.maritima RNase P protein subunit. His-tagged T.maritima RNase P protein subunit was purified on His-Resin columns under (A) denaturing and (B) non-denaturing conditions. After dialysis of the proteins eluted from the columns, 0.5 µg of each protein preparation was separated on a 15% SDS–PA gel and stained with either Coomassie dye (C) or silver (S). (C) Clustal W alignment of the wild-type T.maritima RNase P protein subunit with its recombinant homolog (T.maritima–His) and with the E.coli RNase P protein subunit.