Figure 4.

Temperature dependence of T.maritima RNase P holoenzyme activity. Initial velocities were determined in 5 mM MgCl₂ (in 50 mM HEPES, pH 7.5, 400 mM NH₄Cl) at each respective reaction temperature. The reactions were started by addition of 100 nM of ³²P-labeled substrate RNA. Aliquots were taken at 1 min intervals and separated on 8% denaturing polyacrylamide gels. The amount of cleavage was determined using a phosphoimager.