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Induction of hpaA and aph in P. aeruginosa with 4-HPA requires the presence of intact hpaA genes. Cells were grown overnight in M63 medium with or without supplementation of 5 mM 3-HPA or 4-HPA. Data represent the averages of the results of six independent β-galactosidase activity experiments. pWC011, pWC012, and pWC014 are lacZ fusion plasmids for hpaA-aph, hpaA, and aph, respectively (Fig. 1B); PAK/V and hpaA-/V are vector controls containing pDN19lacΩ.
