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The results of a double-disk diffusion assay show that neomycin resistance in P. aeruginosa can be induced by the presence of 4-HPA in a concentration-dependent manner. This result was not observed for the isogenic aph or hpaA mutant strains or when 3-HPA is used as inducer. The amounts of neomycin and 3- or 4-HPA used in these tests are indicated (see Materials and Methods for details).
