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Detection and differentiation of blaCTX-M genes by PCR-RFLP. Lanes 1 to 5, undigested PCR products; lanes 6 to 10, PstI-PvuII-digested PCR products; lanes 1 and 6, C. freundii 2525 (CTX-M-3); lanes 2 and 7, S. enterica serovar Typhimurium CAS5 (CTX-M-2); lanes 3 and 8, K. ascorbata T861 (KluA); lanes 4 and 9, E. coli (CTX-M-9); lanes 5 and 10, E. coli C600 (TEM-1); lanes M, molecular size marker (pUC18-HaeIII).
