FIG. 5.

Growth of wild-type S. cerevisiae strain A2 and two mutants containing allelic forms of PDR1 (PDR1-12 in KPHJ1) and PDR3 (PDR3-33 in KPHJ2) causing overexpression of Pdr5p in the presence and absence of terbinafine. The yeast strains were inoculated into 2 ml of YPD medium to an OD₆₀₀ of 0.005 and were grown at 30°C for 45 h. The medium was supplemented with 20, 50, or 100 μg of terbinafine per ml dissolved in ethanol. The control culture without terbinafine was supplemented with ethanol. Growth was monitored by measuring the OD₆₀₀.