FIG. 7.

Effects of erg1 alleles expressed from low-copy-number plasmids on terbinafine resistance. S. cerevisiae KLN1 (erg1::URA3) was transformed with the low-copy-number plasmids pML1 carrying the wild-type ERG1 gene, pML2 carrying the erg1 allele for terbinafine resistance of A2M8, and pNS2 carrying erg1 allele NS2 for terbinafine resistance on pRS315. The strains were grown overnight in YPD medium, 5-μl aliquots of the ONCs were diluted to an OD₆₀₀ of 0.1, and serial dilutions (10⁰ to 10⁻³) were spotted on YPD plates without terbinafine (A) and with 100 μg of terbinafine per ml (B) and incubated for 2 days at 30°C.