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. 2001 Feb 15;29(4):872–879. doi: 10.1093/nar/29.4.872

Figure 4.

Figure 4

Complementation of XPF mutant extract with rΔ88ERCC1 and rXPF. Repair reactions (100 µl) were set up with XP-F extract (180 µg protein) and 250 ng of plasmid DNA with a single cisplatin lesion (lane 1) and various amounts of recombinant ERCC1 and/or recombinant XPF (lanes 2–6). Reactions in lanes 2–5 received 3000, 2625, 1500 and 375 ng of ERCC1, respectively. Reactions in lanes 3–6 received 375, 1500, 2625 and 3000 ng of XPF, respectively. Lane 7 is a control reaction with 90 ng of the ERCC1–XPF complex produced in E.coli with a vector encoding both subunits. Excised oligonucleotides are indicated by the brackets on the left. (A) Reactions with extract derived from the CHO UV41 XPF mutant cell line. (B) Reactions with extract derived from the human XP2YO XPF mutant cell line. Dual incision signals are quantified in the lower panels.