TABLE 3.
Summary of pulmonary inflammation or toxicity responses in the BAL of Klebsiella-infected micea
| Group | No. of mice | Neutrophilia % PMNc [mean (SD)]d | Il-6 (pg/ml) [GM (GSD)]e | TNF-α (pg/ml) [GM (GSD)] | Hemoglobin (μg/ml) [mean (SD)] | Protein (μg/ml) [mean (SD)] | K. pneumoniae recoveryb (CFU/ml) [GM (GSD)] |
|---|---|---|---|---|---|---|---|
| Naïve | 23 | 1.8 (1.6) | 20.7 (2.0) | 17.6 (1.6) | 11.8 (9.2) | 47.5 (15.7) | 0 (0) |
| Klebsiella-infected, treated with saline | 17 | 29.9* (26.9) | 67.9 (7.5) | 65 (6.8) | 33.1 (39.4) | 130.5 (169) | 6.9 × 103* (794) |
| Klebsiella-infected, treated with tobramycin (1 mg/kg) | 9 | 23.9 (23.5) | 105 (11.1) | 80 (7.9) | 87.5 (121) | 176.7 (178) | 7.9 × 103* (1259) |
| Klebsiella-infected, treated with G10 (0.4 mg/kg) | 9 | 23.6 (23.4) | 398* (18.5) | 277* (9.4) | 189*† (222) | 357.3* (373) | 4.9 × 104* (5011) |
| Klebsiella-infected, treated with tobramycin (1 mg/kg) + G10 (0.4 mg/kg) | 9 | 53* (24.4) | 684* (6.9) | 525* (4.9) | 63.5 (47) | 235.9 (127) | 3.5 × 106* (63) |
| Klebsiella-infected, treated with tobramycin (10 mg/kg) | 9 | 20.7 (21.1) | 29.4£ (4.6) | 29£ (3.7) | 13.8§ (20.2) | 67.3§ (41.8) | 8.9 × 101£ (501) |
a
Statistical comparisons reference significant differences (P ≤ 0.05) compared to the treatment group indicated by the symbol. The symbols *, †, ‡, §, and £ refer to comparisons made by the Scheffé post hoc procedure.
b
K. pneumoniae recovered from BAL at necropsy.
c
% PMN, percentage of polymorphonuclear leukocytes by differential count.
d
SD, standard deviation.
e
GM, geometric mean; GSD, geometric standard deviation.