Figure 4.

(A) Radiolabelled 13GA oligonucleotide (20 nM) was incubated overnight at room temperature in the presence of 2 µM unlabelled 13GA and 2 µM 31R·31Y target duplex in 50 mM HEPES (pH 7.2), 100 mM NaCl and 10 mM MgCl₂. The sample was run on a TGGE gel with a linear temperature gradient between 20 and 65°C. The arrows indicate the corresponding species. (B) Radiolabelled 13GA oligonucleotide (20 nM) was incubated overnight at 37°C in the presence of 2 µM 31R·31Y target duplex in the same buffer as in (A) and was run with a temperature gradient ranging between 30 and 75°C. The arrows indicate the corresponding species.