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. 2003 Dec;185(24):7184–7192. doi: 10.1128/JB.185.24.7184-7192.2003

FIG. 3.

FIG. 3.

Transcriptional analysis of the croRS locus. (A) The positions of oligonucleotides P49, P51, and P76 used for primer extension and of the DNA fragments used as probes for the Northern blot hybridizations (solid boxes) are indicated above the map of the cluster. Open boxes below the map represent the DNA fragments tested for promoter activity in pTCV-lac. (B) Northern blot analysis of total RNA with probes generated from internal fragments of croR (lanes 1 to 4), croS (lanes 5 to 8), and serS (lanes 9 to 12). Total RNA was extracted from E. faecalis JH2-2 grown in the absence (lanes 1, 5, and 9) or presence (lanes 2, 6, and 10) of ceftriaxone (1,000 μg/ml). RNA from JH2-2ΔcroS (lanes 3, 7, and 11) and JH2-2ΔcroRS (lanes 4, 8, and 12) was extracted from bacteria grown in the absence of ceftriaxone.