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Southern blot analysis of narG and narX insertional mutants. Chromosomal DNA from strains used to produce the RVW1 (A), RVW2 (B), and RVW3 (C) mutants was isolated from the wild type (lanes 1), single crossovers containing two copies of the nar gene (lanes 2), and double crossovers containing aphI inserted into nar (lanes 3). DNA was cut with KpnI (A and B) or SmaI (C) and probed with a probe specific for either narG (A), narX (B), or narK2 (C).
