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Anaerobic growth of an E. coli nar mutant with M. tuberculosis narGHJI1. E. coli was grown anaerobically in M9 medium containing glycerol and 20 mM NaNO3. Symbols: ▴, wild type; ▪, E. coli JCB4023 with pNarGHJI1 (complementation mutant); •, E. coli JCB4023 with pPE207 (mutant with only the vector).
