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Nitrite production by an E. coli mutant with M. tuberculosis narK2. E. coli was grown anaerobically in LB medium containing 20 mM NaNO3. Symbols: ▴, wild type; ▪, E. coli JCB4018 pTSJ4 (complementation mutant); •, E. coli JCB4018 with pSK (mutant with only the vector).