TABLE 1.
Oligonucleotide primers used in this study
| Primer | Sequence (5′→3′)a | Purpose in this studyb |
|---|---|---|
| p11 | ACTACGCCGACAACACCAAGTTCG | Probe for narG |
| p12 | TGTTCTGCTGGGTTCGGTCGTGGTA | Probe for narG |
| p13 | TGCTGCTGGTGGCGACCGCGCTGGTCGCGT | Cloning narX |
| p14 | GCGCGGACTTGTTCGACGAGTAGACGTGT | Cloning narX |
| p19 | GTGAGCGGGTACCAATTTCACACAGG | Cloning lacZ |
| p20 | GGCCTGCCCGGGTATTATTATTTTTG | Cloning lacZ |
| p23 | GGTGATCGCCATGGTCGACCTGCAGG | Cloning aphI |
| p24 | TAATGCTCTGCCATGGTTACAACCAA | Cloning aphI |
| p27 | ATGCGCCTCGGTGCTGCTGACCTACCCGAA | Probe for narX |
| p28 | GGATCGGCACGGCGCAGCTCAGAGACCGTG | Probe for narX |
| p46 | CTGGATTGCGGATCTAGAGCGAACCCTCAA | Construction of pMP101 |
| p47 | ACGGTCACGGGGGTACCCTCCTCGTCATGA | Construction of pMP101 |
| p48 | TCCCCTTTCTAGAGGCGACCAGGCTCAGCT | Construction of pMP102 |
| p49 | TCATCGACAGGTACCGGGGTCTCGGACTCC | Construction of pMP102 |
| p51 | CAGCTCCTGGATCCGGCTGCCGGTCCGTGG | Cloning narK2 |
| p58 | ACTCGAGTGGCGAACGGGTGAGTAACACGT | RT-PCR of 16S rRNA |
| p59 | AGGCCGTCACCCCACCAACAAGCTGATAGG | RT-PCR of 16S rRNA |
| p60 | ACTACGCCGACAACACCAAGTTCGCCGACG | RT-PCR of narG |
| p61 | AGCGGCGCACATAGTCGACAAAGAACGGAA | RT-PCR of narG |
| p62 | ATTGGTGGGACGTGGTGTGGCAATGCGCCT | RT-PCR of narX |
| p63 | GACCGTCGATGTGGGCCAGCAATTCCTCTG | RT-PCR of narX |
| p66 | TGCTTCGTGATGCACCCTACTTTCGGCCCA | RT-PCR of narK2 |
| p67 | CCGCCGAACACGATCGCGTACAGAAACGAC | RT-PCR of narK2 |
| p68 | CCAAGTCGGACAAGCTTCGGGCGACCGAGA | Cloning narK2 |
a
Mismatches used to create restriction sites are indicated by underlining.
b
RT-PCR, reverse transcriptase PCR.