TABLE 2.
Production of nitrite in whole-cell culture by M. tuberculosis and M. bovis
| Mycobacterium | Treatmenta | Nitrite concnb
|
||
|---|---|---|---|---|
| Aerobicc | NRP-1d | SSPe | ||
| M. tuberculosis | 130 ± 23 | 1,100 ± 80 | 2,600 ± 430 | |
| 400 μM Mo | 120 ± 6 | 780 ± 55 | ND | |
| 100 μM W | 9 ± 1 | 120 ± 9 | ND | |
| 400 μM Mo + 100 μM W | 23 ± 3 | 500 ± 5 | ND | |
| 40 μM azide | 3 ± 1 | 16 ± 7 | ND | |
| M. bovis | 13 ± 1 | 5 ± 2 | 180 ± 52 | |
| M. bovis BCG | 7 ± 1 | 2 ± 1 | 11 ± 5 | |
a
Treatment with the ions molybdate (Mo) and tungstate (W) and sodium azide.
b
Mean nitrite concentration (micromolar) ± standard deviation.
c
After 112 h of growth (OD580 of ∼0.4).
d
After 112 h, approximately 45 h in NRP-1 (OD580 of 0.1).
e
After 280 h of growth (OD580 > 1.0). ND, not done.