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Nitrate reductase activity in cell-free sonicates
| Culture treatment | Nitrite reductase activitya
|
||
|---|---|---|---|
| Aerobicb | NRP-1c | SSPd | |
| No nitrate | 20 ± 1 | 22 ± 5 | 9 ± 1 |
| 5 mM NO3− | 21 ± 1 | 18 ± 3 | 6 ± 1 |
| 20 mM NO3− | 21 ± 2 | 22 ± 4 | ND |
Mean nitrite reductase activity (in nanomoles of NO2− per minute per milligram of protein) ± standard deviation.
After 112 h of growth (OD580 of ∼0.4).
After 112 h, approximately 45 h in NRP-1 (OD580 of 0.1).
After 280 h of growth (OD580 > 1.0). ND, not done.
