TABLE 5.

Nitrate reductase activity of narG, narX, and narK2 mutants in the cell-free assay

Strain	Genotype	Nitrate reductase activitya
		Aerobicb	NRP-1c	SSPd
RVW1	ΔnarG	0.02 ± 0.01	0.02 ± 0.01	0.03 ± 0.02
RVW2	ΔnarX	18 ± 2	21 ± 2	8 ± 2
RVW3	ΔnarK2	25 ± 4	23 ± 2	6 ± 1

^aMean nitrite reductase activity (in nanomoles of NO₂⁻ per minute per milligram of protein) ± standard deviation.

^bAfter 112 h of growth (OD₅₈₀ of ∼0.4).

^cAfter 112 h, approximately 45 h in NRP-1 (OD₅₈₀ of 0.1).

^dAfter 280 h of growth (OD₅₈₀ > 1.0).