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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Microvasc Res. 2010 Jun 21;80(3):394–401. doi: 10.1016/j.mvr.2010.06.005

Fig. 2.

Fig. 2

Fluorescent labeling of the endothelial cell (EC) glycocalyx. (A) Brightfield image of a post-capillary venule (diameter = 40.7 μm). The plasma membrane of the EC was taken as the outermost edge of the dark refractive band between the EC and plasma layer. (B) Fluorescence image 10-min following proximal micropipette infusion of BS1-Alexa lectin. In this example fluorescence was confined to the left microvessel wall due to heterogeneity of network perfusion. A measurement line was drawn along the left EC wall and fluorescence intensity was averaged over an area within 0.5 μm on either side of the measurement line.