Table 1.
Statistics of vessel diameters and curve fits determining the boundary of the glycocalyx and the diffusion coefficient of FITC
| Treatment: | Control | Heparinase | Chondroitinase | Hyaluronidase | Enzyme mix |
fMLP | |
|---|---|---|---|---|---|---|---|
| (A) BS1 Cleavage assay |
N | 12 | 8 | 8 | 12 | 11 | |
| Diameter (μm) | 42.6 ± 6.19 |
45.4 ± 8.1 |
47.2 ± 8.3 |
44.6 ± 7.1 |
42. 1 ± 11.4 |
||
| (B) Sigmoidal fits of FITC-Dx70 radial intensity * |
N | 77 | 14 | 17 | 16 | 17 | 13 |
| Diameter (μm) | 38.3 ± 7.58 |
37.8 ± 10.3 |
40.0 ± 8.5 |
39.9 ± 6.2 |
36.9 ± 5.7 |
36.2 ± 5.2 |
|
| (C) Intensity- distance-time fits for diffusion coefficient calculation |
N | 10 | 7 | 7 | 8 | 9 | |
| Diameter (μm) | 25.5 ±5.9 |
27.7 ± 8.1 |
38.3 ± 10.8 |
35.7 ± 13.3 |
27.4 ± 7.4 |
||
| RMS error (%) DDx70 |
35.0 ± 0.9 |
33.8 ± 0.2% |
33.9 ± 0.2 |
34.5 ± 0.6 |
34.2 ± 0.4 |
||
| RMS error (%) D173 |
33.7 ±0.2% |
33.6 ±0.1% |
33.5 ±0.1% |
33.8 ±0.2% |
33.7 ±0.2% |
Data are Mean ± SD
In each case, all treatments were not statistically significant from control for diameter and goodness of of of fit.
*
For all sigmoidal fits, R2=0.9998±0.0001 SD