Skip to main content
. Author manuscript; available in PMC: 2010 Oct 22.
Published in final edited form as: Cell. 2007 Aug 10;130(3):427–439. doi: 10.1016/j.cell.2007.06.044

Figure 2. Mutant R120GCryAB induces the HSP stress response pathway.

Figure 2

(A, B) Representative Western blot experiments of (A) supernatants or (B) insoluble fractions (pellets) from heart extracts of 6 month old NTg, hCryAB Tg, hR120GCryAB Low Tg and hR120GCryAB High Tg mice, immunoblotted with anti-Hsp25, -Hsp70, and -Hsp90 antibodies. Each lane represents an individual animal (3 animals/group).

(C, D) Densitometry values are represented as relative intensities in mean arbitrary units calculated from the Western blots shown in Figures 2A and 2B, respectively (*p<0.05, **p<0.01, †p<0.001).

(E, F) Northern blots show that Hsp25 transcripts are significantly increased in hR120GCryAB hearts at 3 and 6 month old animals (*p<0.05).