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. Author manuscript; available in PMC: 2010 Oct 22.
Published in final edited form as: Cell. 2007 Aug 10;130(3):427–439. doi: 10.1016/j.cell.2007.06.044

Figure 3. Enzyme activity and protein expression of glutathione peroxidase-1 (GPx-1) and catalase at 6 months.

Figure 3

(A, B) Mutant hR120GCryAB Tg High overexpression enhances the activities of GPx-1 and catalase (*p 0.05) in 6 month old hearts.

(C, D) At 6 months, protein expression for GPx-1 is unchanged but catalase was increased in hR120GCryAB High animals compared with NTg, hCryAB Tg and hR120GCryAB Low Tg animals. Moderate increase in GPx activity (panel A) without a commensurate increase in GPx protein expression may reflect the translational limitations of available selenium, which is not standardized in chows, and/or of the translational cofactors required for selenoprotein synthesis (Handy et al., 2006). Each lane represents an individual animal (3 animals/group).

(E) Densitometry of Western blots presented in Figure 3C reveals that catalase level was increased by ~ 5 fold in hR120GCryAB High Tg compared with the other groups (**p<0.02).

(F) Northern blot analysis using radio-labeled cDNA probes against GPx-3 and catalase (Cat). Total RNA was harvested from NTg, hCryAB Tg and hR120GCryAB High Tg at either 3 or 6 months.

(G, H) Densitometry analysis of Northern blots of Figure 3F expressed in arbitrary units shows ~ 2–3 fold increases for GPx-3 (G) and catalase (H), in both 3 and 6 month old hR120GCryAB High Tg hearts (*p<0.05). Each lane represents an individual animal (3 animals/group).