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. Author manuscript; available in PMC: 2010 Oct 22.
Published in final edited form as: Cell. 2007 Aug 10;130(3):427–439. doi: 10.1016/j.cell.2007.06.044

Figure 5. R120GCryAB Overexpression Promotes Co-localization and Novel Interactions between G6PD and Hsp25 in Protein Aggregates.

Figure 5

(A) Representative Westerns of supernatant fractions from heart homogenate after co-immunoprecipitation were performed and probed with anti-G6PD, anti-CryAB and anti-Hsp25 antibodies.

(B) Densitometry analysis of immunoblots indicates significant interactions among CryAB, Hsp25 and G6PD in the hR120GCryAB High Tg group. G6PD/CryAB (panels A & B-a); CryAB/G6PD (panels A & B-b); G6PD/Hsp25 (panels A & B-c); and Hsp25/G6PD (panels A & B-d). (*p ≤ 0.05, **p<0.01 compared with NTg control).

(C) Protein aggregates in hR120GCryAB High Tg mice contain moderate levels of both CryAB (b, shown in green) and Hsp25 at 6 months (d, red). G6PD is also expressed more diffusely (f, blue) but appears to be slightly concentrated in or around the aggregates (h, three images merged). Inset arrow (panel h) is a higher magnification of a representative myocardial section contained in the squares (panels b, d, f, and h). No aggregates are seen in transgenic mice expressing the wild type version of human CryAB (panels a, c, e, and g).