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PKR is differentially activated by distinct TLRs. Primary thioglycolate-elicited macrophages were treated for the indicated times with either E. coli LPS (250 ng/ml), P3C (500 ng/ml), or p(I ⋅ C) (50 µg/ml). At the indicated times, whole-cell lysates were subjected to Western analysis and probed with antibodies directed against phosphorylated or total protein populations. These data are representative of 6 independent experiments.
