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Tetramer analyses. Subjects 1 and 4 were tested for CD4+ T cells with allorecognition toward parathyroid donor oligopeptides (from the HLA-DRB1 allele not shared with the recipient) presented within recipient-type MHC class II tetramers (“positive”). “Negative” tetramers contained an antigenically irrelevant peptide. Percentages of tetramer-positive cells are shown, gated on CD4+ T cells.
