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. 2010 Aug 23;285(44):33589–33601. doi: 10.1074/jbc.M110.117150

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Construction of MS2-Pab1 fusion proteins. a, shown is a schematic drawing of FLAG-MS2-Pab1p wild type and deletion mutant fusion proteins. The black box represents the FLAG tag. The white box appears to show MS2. The numbered boxes indicate the RRM domains of Pab1p, and the shaded box labeled with a C represents the carboxyl terminal domain of the poly(A)-binding protein. b, shown are expression levels of the FLAG-MS2-Pab1p fusion proteins. W303 cells harboring the indicated FLAG-MS2-Pab1p fusion genes, and pIT2069 (AUG-FLAG-MS2, (AUG)), pIT2071 (No-AUG-FLAG-MS2, (No-AUG)), or pIT2073 (SL-AUG-FLAG-MS2, (SL-AUG)) plasmids were grown on SC-UraLeu medium containing 2% galactose. Samples were analyzed by Western blotting using anti-FLAG antibodies.