FIGURE 4.

Tethering of Pab1p stabilizes non-translated mRNAs without affecting association of the mRNA with the ribosome. W303 cells were co-transformed with the indicated reporter plasmids and a control p415TEF1p-MS2 plasmid or a plasmid that encodes the MS2-Pab1–34Cp fusion protein. Polysome analysis was then performed on cell extracts as described previously. RNA samples prepared from each fraction were analyzed using Northern blotting with a DIG labeled FLAG probe (5′-CTTGTCATCGTCGTCCTTGTAGTCCATACTAGT-3′).