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. 2010 Aug 23;285(44):33589–33601. doi: 10.1074/jbc.M110.117150

FIGURE 6.

FIGURE 6.

Stabilization of non-translated mRNA by tethered Pab1p-34Cp in an xrn1Δ 5′ to 3′ mRNA degradation mutant requires a poly(A) tail. The cells were grown in SC-Gal UraLeu medium, and RNA samples were prepared and analyzed by Northern blotting as described in Fig. 3a. Relative quantities are shown as the mean values of three independent experiments. The half-lives of mRNAs are shown as the mean values with S.D. a, W303xrn1Δ cells harboring pIT2071 (No-AUG-FLAG-MS) or pIT2070 (No-AUG-FLAG) were transformed with a control p415TEF1p-FLAG-MS2 plasmid or with a plasmid that encodes the FLAG-MS2-Pab1–34Cp fusion protein. b, W303xrn1Δ cells harboring pIT2069 (AUG-FLAG-MS) or pIT2068 (AUG-FLAG) were transformed with control p415TEF1p-FLAG-MS2 plasmid or with a plasmid that encodes the FLAG-MS2-Pab1–34Cp fusion protein. c, W303xrn1Δ cells harboring pIT2085 (No-AUG-FLAG-MS2-Rz) or pIT2084 (No-AUG-FLAG-Rz) were transformed with a control p415TEF1p-FLAG-MS2 plasmid or with a plasmid that encodes the FLAG-MS2-Pab1–34Cp fusion protein.