FIGURE 8.

Tethering of Pab1p-34C stabilizes mRNAs that contain a stable stem-loop structure at the 5′-UTR. The cells were grown in SC-Gal UraLeu medium. RNA samples were prepared, and mRNA stability was measured as described in Fig. 3a. Relative quantities are shown as the mean values of three independent experiments. The half-lives of mRNAs are shown as the mean values with S.D. a, W303 cells harboring the pIT2073 (SL-AUG-FLAG-MS2) plasmid were transformed with a control p415TEF1p-FLAG-MS2 plasmid or with a plasmid that encodes the FLAG-MS2-Pab1–34Cp fusion protein. b, W303ski2Δ cells harboring pIT2073 (SL-AUG-FLAG-MS2) were transformed with a control p415TEF1p-FLAG-MS2 plasmid or with a plasmid that encodes the FLAG-MS2-Pab1–34Cp fusion protein.