TABLE 1.
Yeast strains and plasmids used in this study
| Strain/plasmids | Genotype [plasmid] (plasmid number) | Source |
|---|---|---|
| Strains | ||
| W303-1a | MATa ade2 his3 leu2 trp1 ura3 can1 | Laboratory stock |
| YAS2071 | MATa ade2 his3 leu2 trp1 ura3tif4631::LEU2 tif4632::ura3 pep4::HIS3 ptif4631-ΔN300TRP1CEN | Ref. 31 |
| YIT2007 | W303-1a ski2Δ::kanMX | Ref. 40 |
| YIT2008 | W303-1a xrn1Δ::natMX | This study |
| Plasmids | ||
| p416GAL1 | p416GAL1p URA3 CEN | Ref. 41 |
| p415TEF1 | p415TEF1p LEU2 CEN | Ref. 42 |
| pIT2067 | p416GAL1p-AUG URA3 CEN | This study |
| pIT2068 | p416GAL1p-AUG-FLAG-MPT4ΔN-PGK1(3′-UTR) URA3 CEN | This study |
| pIT2069 | p416GAL1p-AUG-FLAG-MPT4ΔN-PGK1(3′-UTR)-MS2 URA3 CEN | This study |
| pIT2070 | p416GAL1p-No-AUG-FLAG-MPT4ΔN-PGK1(3′-UTR) URA3 CEN | This study |
| pIT2071 | p416GAL1p-No-AUG-FLAG-MPT4ΔN-PGK1(3′-UTR)-MS2 URA3 CEN | This study |
| pIT2072 | p416GAL1p-SL-AUG-FLAG-MPT4ΔN-PGK1(3′-UTR) URA3 CEN | This study |
| pIT2073 | p416GAL1p-SL-AUG-FLAG-MPT4ΔN-PGK1(3′-UTR)-MS2 URA3 CEN | This study |
| pIT2074 | p416GAL1p-AUG-MPT4ΔN-FLAG-PGK1(3′-UTR)-MS2 URA3 CEN | This study |
| pIT2075 | p416GAL1p-No-AUG-MPT4ΔN-FLAG-PGK1(3′-UTR)-MS2 URA3 CEN | This study |
| pIT2076 | p416GAL1p-SL-AUG-MPT4ΔN-FLAG-PGK1(3′-UTR)-MS2 URA3 CEN | This study |
| pIT2077 | p415TEF1p-FLAG-PAB1 LEU2 CEN | This study |
| pIT2078 | p415TEF1p-FLAG-MS2-PAB1 LEU2 CEN | This study |
| pIT2079 | p415TEF1p-FLAG-MS2-Pab1–34C LEU2 CEN | This study |
| pIT2080 | p415TEF1p-FLAG-MS2-Pab1–4C LEU2 CEN | This study |
| pIT2081 | p415TEF1p-FLAG-MS2-Pab1-C LEU2 CEN | This study |
| pIT2082 | p416GAL1p-No-AUG-FLAG-MPT4ΔN-PGK1(3′UTR)-Rz URA3 CEN | This study |
| pIT2083 | p416GAL1p-No-AUG-FLAG-MPT4ΔN-PGK1(3′UTR)-MS2-Rz URA3 CEN | This study |
| pIT2084 | p416GAL1p-Rz-No-AUG-FLAG-MPT4ΔN-PGK1(3′UTR)-Rz URA3 CEN | This study |
| pIT2085 | p416GAL1p-Rz-No-AUG-FLAG-MPT4ΔN-PGK1(3′UTR)-MS2-Rz URA3 CEN | This study |
| pIT2086 | p415TEF1p-MS2-PAB1 LEU2 CEN | This study |
| pIT2087 | p415TEF1p-MS2-Pab1–34C LEU2 CEN | This study |
| pIT2088 | p415TEF1p-MS2-Pab1–4C LEU2 CEN | This study |
| pIT2089 | p415TEF1p-MS2-Pab1-C LEU2 CEN | This study |