TABLE 2.
List of oligonucleotides
nt, nucleotides.
| Name description | Sequences |
|---|---|
| OTS017 MPT4ΔN (380–822 nt) Forward | 5′-GCTCTAGAATGGACTACAAGGACGACGACGACAAGCTTGCTGAAATTGCTGAAGACGC-3′ |
| OIT934 MPT4ΔN (380–822 nt) Reverse | 5′-GCGGGATCCTTAAGCCAAAGATGGCAAGTTAGAAACGTCAATG-3′ |
| OIT932 AUG to SpeI restriction | 5′-GACTAGTGCAAATTCCAAGTACTCCTTGGTCTTC-3′ |
| OIT933 AUG to SpeI restriction | 5′-GACTAGTCACTTTTGTTGAATCTAACACTAGAAAGAACTTCG-3′ |
| OIT557 PGK1 3′-UTR forward | 5′-GGGATCCTAAATTGAATTGAATTGAAATCGATAG-3′ |
| OIT558 PGK1 3′-UTR reverse | 5′-GGGAATTCCGATTGACCAATATATGTCTCTGAATGCC-3′ |
| OKK130 XhoI site top | 5′-CCCCATCCTTTACCTCGAGCTAAAATAATAG-3′ |
| OKK131 XhoI site bottom | 5′-CTATTATTTAGCTCGAGGTAAAGGATGGGG-3′ |
| OIT949 MS2 binding sites top | 5′-ATCGAGTAACAAGAGGATCACCCTTGTCTGCAGGTCGACTCAAGAAAACTTGAGGATCACCCAAGT-3′ |
| OIT950 MS2 binding sites bottom | 5′-CGAACTTGGGTGATCCTCAAGTTTTCTTGAGTCGACCTGCAGACAAGGGTGATCCTCTTGTTAC-3′ |
| OIT953 No-AUG-MPT4ΔN (380–822 nt) forward | 5′-GCTCTAGATACGACTACAAGGACGACGACGACAAGCTTGCTGAAATTGCTGAAGACGC-3′ |
| OTS10 MPT4ΔN (380–822 nt)-FLAG reverse | 5′-CGGGATCCTTACTTGTCGTCGTCGTCCTTGTAGTCAGCCAAAGATGGCAAGTTAGAAACGTCAATGTTACGGTTCTTTTGAACGGTGTTAGCAGAGTTA-3′ |
| OIT1153 Stem-loop | 5′-CTAGCGATATCCCGTGGAGGGGCGCGTGGTGGCGGCTGCAGCCGCCACCACGCGCCCCTCCACGGGATATCG-3′ |
| OTS034 SpeI site top | 5′-TAGACTATTATTTATCTTTTAAACTAGTTGATTATTAAGATTTTTATTA-3′ |
| OTS035 SpeI site bottom | 5′-TAATAAAAATCTTAATAATCAACTAGTTTAAAAGATAAATAATAGTCTA-3′ |
| OIT1181 Ribozyme top SpeI and XbaI | 5′-CTAGCCCTGTCACCGGATGTGTTTTCCGGTCTGATGAGTCCGTGAGGACGAAACAGGA-3′ |
| OIT1182 Ribozyme bottom SpeI and XbaI | 5′-CTAGTCCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAACACATCCGGTGACAGGG-3′ |
| OIT627 Pab1 forward | 5′-GCTCTAGAATGGCTGATATTACTGATAAGACAGC-3′ |
| OIT884 Pab1 reverse | 5′-ACGCGTCGACGTAGGGAAGTAGGTGATTACATAGAGC-3′ |
| OIT670 MS2 protein 5′ | 5′-GCTCTAGAATGGACTACAAGGACGACGATGACAAGGCTTCTAACTTTACTCAGTTCG-3′ |
| OIT630 MS2 protein reverse | 5′-GCTCTAGAGTAGATGCCGGAGTTTGCTGCGATTGCTGAGGG-3′ |
| GTD029 Pab1–34C forward | 5′-GCTCTAGAGACTCTCAATTGGAAGAGACTAA-3′ |
| OIT883 Pab1–4C forward | 5′-CTCTAGAGCTTACAGATTGGAAAAAATGGCC-3′ |
| GTD038 Pab1-C forward | 5′-GCTCTAGAGCCGGTATGCCAGGTCAAT–3′ |