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. 2010 Aug 19;285(44):33602–33613. doi: 10.1074/jbc.M110.135640

FIGURE 3.

FIGURE 3.

Luciferase reporter assay reveals that PCFT promoter activity is modulated by NRF-1 levels. A–C, HeLa cells were transiently co-transfected with plasmids: 1) pRL-O Renilla; 2) pGL3-271 bp PCFT promoter construct; and 3) various amounts (ng) of NRF-1 WT (A), NRF-1 VP16 (B), or NRF-1 DN (C). Luciferase activity was then determined as detailed under “Experimental Procedures.” Results are presented as a luciferase/Renilla ratio (%), normalized to the pGL3-271-bp construct along with the corresponding amount of pCDNA empty vector assigned 100% activity. D, luciferase reporter assay in HeLa cells following co-transfection with plasmids: 1) pRL-O Renilla plasmid; 2) 2.5 ng of NRF-1 WT or NRF-1 DN; 3) PCFT promoter constructs pGL3-3.1 kb, -271 bp, or -157 bp. Results are presented as a luciferase/Renilla ratio (%), normalized to the corresponding PCFT promoter construct along with the 2.5 ng of pCDNA empty vector assigned 100% activity. Results shown are the mean ± S.D of three independent experiments performed in duplicates.