FIGURE 7.

Conserved targeting of the β-oxidation gene CPTI in the cholesterol auxotrophs Drosophila. A, evolutionary conservation of the predicted miR-33 binding site in the 3′ UTR of the Drosophila, Anopheles, and C. intestinalis CPT1 transcripts. B, reporter gene experiment using the 3′ UTR of the CPT1 transcript (CPT1) or mutants containing site-directed mutations of the predicted miR-33 seed binding region (CPT1 MUT) were cotransfected with pre-miR-33 or a pre-miR control, and evaluated identically to the experiment described in the legend to Fig. 2B. The asterisk denotes p < 0.05 in Student's t test (n = 3). Error bars denote S.D.