FIGURE 4.

Adora gene expression in the adult testis. A, organization of the mouse Adora3 gene, present on chromosome 3: 105673825–105726959. Approximate scale indicated by scale bar. Numbers at the right indicate exon useages. B, PCR for full-length Adora3 transcripts in the testis; Adora3i1 (black arrowhead) and Adora3i2 (white arrowhead) were amplified. C, relative expression of Adora transcripts in the testis reported by quantitative RT-PCR of testicular cDNA with isoform-specific primers for Adora1, Adora2a, Adora2b, Adora3i2, and primer for the conserved coding region of Adora3i1 and Adora3i3. PCR products were confirmed by sequencing and predicted product length was verified by agarose gel electrophoresis (lower panel).